Effect of arsenite on the proteome of earthworms Eisenia fetida

Arsenic (As) is broadly distributed due to natural and anthropogenic sources, it toxic organisms. This study aimed investigate the proteomic response in earthworm exposed As3+. Earthworms were As3+ soil at 5–80 mg kg?1, samples collected after 60 days exposure. Two-dimensional electrophoresis (2-DE) was used separate proteins homogenate, then differentially expressed (DEPs) identified using MALDI-TOF/TOF-MS analysis. After2-DE, 36 DEPs found 24 of them successfully identified. 79.2% upregulated compared control group. The maximum fold change reached 53.8 spot 3108 80 kg?1 As Two not group but treated groups. Proteins grouped into metabolism, signal transduction, stress-related, transport, regulation, predicted/hypothetical protein categories based on their function. protein—protein interaction between indicated that serum albumin (ALB) very important, related 6 other proteins. verified by western blot, results agreement with analyses. identification induced or repressed because earthworms helpful explore underlying mechanisms arsenic ecotoxicity.